Evaluating Weak versus Tight Coomassie Blue-Protein Binding
نویسندگان
چکیده
منابع مشابه
The solubility of Coomassie blue protein-dye complexes.
Coomassie blue protein dye-binding assays are commonly used for quantitation of protein. They exploit the interaction between protein and Coomassie brilliant blue (CBB) G-250, the latter being solubilized in either ethanol/phosphoric acid (1) or perchloric acid (2). The Bradford assay (1) is the method of choice. Potential problems with these assays include non-linearity, a variable response to...
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The binding of CBB to BSA was investigated under imitated physiological conditions employing different optical spectroscopic techniques viz., fluorescence emission, UV-vis absorption and FTIR. Fluorescence quenching data obtained at different temperatures suggested the presence of dynamic type of quenching mechanism. The values of K and n for CBB-BSA system were calculated and found to be 4.20 ...
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The demonstration by Hamilton et al. (1948) and Werko et al. (1949) that the analysis of timeconcentration curves of intravenously injected Evans Blue (T-1824) gave estimates of the cardiac output in man that corresponded closely with those obtained by the direct Fick method stimulated a widespread use of the dye method. However, those properties of Evans Blue that led Dawson et al. (1920) to s...
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This simple method of centrifugal analysis for total protein in human breast milk is based on the change in the wavelength of the absorbance maximum of Coomassie Brilliant Blue G-250 when the dye is bound to protein. Within-run and between-day CVs were 3.8% and 4.8%, respectively. Compared with a micro-Kjeldahl method for determination of total nitrogen, the coefficient of correlation was 0.99.
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The Coomassie Brilliant Blue G-250 method for protein in urine has been evaluated for analytical accuracy and clinical applicability. Extremely simple to perform, the test exhibits good precision and sensitivity. The color developed per gram of protein is protein-type dependent, so no single protein standard is completely satisfactory. Color intensity is linearly related to concentration up to ...
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ژورنال
عنوان ژورنال: Journal of Chemical Education
سال: 2004
ISSN: 0021-9584,1938-1328
DOI: 10.1021/ed081p335.2